通过同源重组,插入了一个floxed neo cassette,替换了3-6号外显子。通过RT-PCR分析,证实了肝脏和肾脏中没有RNA表达。通过 Western blot 检测,确认了肝脏提取物中没有蛋白质表达。(来源:J:157029)
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A floxed neo cassette was inserted by homologous recombination replacing exons 3-6. Absence of RNA expression in the liver and kidneys was confirmed by RT-PCR analysis. Absence of protein was confirmed by western blot of liver extracts. (J:157029)