This allele was generated by inserting an EGFP-IRES-loxP-FRT sequence into the 5' UTR of the En2 locus immediately upstream of the ATG and a loxP site into the intron. An FRT-flanked neo cassette was removed by flp mediated recombination. Cre recombination deleted the En2 sequence, producing a null allele with a GFP reporter. (J:156169)