Tg(Aqp4-cre/ERT2)55-27Fwp

The creERT2 expression cassette, preceded by a rabbit beta-globin DNA encompassing intron 2 (beta-globin intervening sequences [bGIVS]) and followed by the SV40 polyA signal and a PGK/Tn5-driven neomycin resistance cassette flanked by FRT sites (neo) was inserted by homologous recombination at the transcription initiation site of the Aqp4 gene located in BAC RP23-189N2. The linearized vector was injected into pronuclei of fertilized FVB/N eggs. In line 55-27, cre/ERT2 mRNA was detected in similar brain regions as Aqp4 but at much lower concentrations. (J:156087)