Tg(Thy1-APPSweArc)BLngn

A human amyloid beta (A4) precursor protein cDNA sequence (APP695) was altered to include the APP KM670/671NL (Swedish) + E693G (Arctic) Familial Alzheimer's Disease (FAD) mutations. The resultant APPArcSwe construct was then placed downstream of the mouse thymus cell antigen 1, theta [Thy1] promoter (via insertion into the ATG start site in exon 2 of the mouse Thy1 locus in the Thy-1323-cassette). The resulting transgene was microinjected into (C57BL/6xCBA)F1 pronuclear oocytes. Mice from founder line B had both Swedish and Arctic mutations; these tg-APP^ArcSwe transgenic mice were subsequently backcrossed to C57BL/6JBomTac. (J:105265)