一个新霉素选择 cassette 替换了 ex1 和起始密码子。RT-PCR 和 Western blot 的结果显示 mRNA 几乎缺失,而蛋白质也完全不存在。(来源:J:155749)
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A neomycin selection cassette replaced exon 1 and its start codon. RT-PCR and Western blot analysis indicated the near absence of mRNA and the absence of protein respectively. (J:155749)