部分exon1被替换了新插入的neo cassette,通过脑组织的 Western blot 分析确认了蛋白表达缺失(来源:J:155607, J:155724)。
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A portion of exon 1 was replaced with a neo cassette. The absence of protein expression was confirmed by western blot analysis on brain extracts. (J:155607, J:155724)