一段3.8kb的基因组片段,包括exon 1,通过同源重组被替换为pgk-puro抗性元件。 Western blot和RT-PCR的结果证实了纯合MEFs中没有表达。(来源:J:155001)
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A 3.8 kb genomic fragment including exon 1 was replaced with a pgk-puro resistance cassette via homologous recombination. Western blot and RT-PCR analysis confirmed the absence of expression in homozygous MEFs. (J:155001)