Opa1^M1Bewi

ENU induced a G to A transition in intron 10 (c.1065+5 G->A) that disrupts the splicing and produce a transcript that lacks the 27 amino acids. These amino acids are coded by exon 10 and are an integral part of the GTPase domain. Western blot did not detect a protein product produced from this allele and a 50% reduction in endogenous protein levels in heterozygous mice. (J:154966)