一个8.3千碱基的mouse insulin promoter(Ins1,从转录起始点-8280到+12的位置)驱动编码cre重组酶的突变型mouse estrogen receptor的ligand结合域的cDNA。额外添加了2.1千碱基的人生长激素(hGH)基因作为转录终止信号。12.5千碱基的构建物被纯化后,注入了CD1小鼠的受精卵核。线1成功建立。(来源:J:169727)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count