The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 760bp between positions 5557663-5558422 of Chromosome 5 (Build GRCm39). This deletion replaces the entire coding sequence residing only within exon 5 with a lacZ cassette and a loxP flanked neomycin-selection cassette. The deletion includes 28 bps upstream of the translational start site and 21 bps downstream of the stop codon. (J:136110, J:279934)