germ line(生殖细胞)通过flp介导的重组移除了LacZ基因和neo cassette,保留了exon 2的内含子。 Western blot分析在纯合子的提取物中确认了蛋白质表达减少了35%。(来源:J:154794)
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Germ line, flp mediated recombination removes the LacZ gene and neo cassette leaving exon 2 floxed. A 35% reduction in protein expression was confirmed by western blot analysis on extracts from homozygotes. (J:154794)