Stx3^{Gt(EUCE320f12)Hmgu}

A gene trap cassette flanked by inversely oriented pairs of the heterotypic FRT/F3 and lox511/loxP recombinase target sequences was inserted in intron 1 of the gene. Expression of FLP recombinase will invert the gene trap from its sense (mutagenic) orientation to antisense, allowing expression of the gene (floxed allele); subsequent Cre expression will reinduce the mutation by the reinversion of gene trap to its sense orientation, generating a knockout allele. (J:275367)