在基因的1号外显子和2号外显子之间插入了基因捕获载体,通过PCR分析、 Western blotting 和免疫荧光实验,结果显示这些来自纯合小鼠的成纤维细胞中未检测到编码的mRNA或蛋白质。(来源:J:228049)
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A gene-trap cassette inserted between exons 1 and 2 of the gene. PCR analysis, Western blotting and immunofluorescence confirmed that no encoded mRNA or protein was detectable in fibroblast cells isolated from homozygous mice. (J:228049)