通过定点突变技术,用PCR替换了BH3域(在第5外显子),用的是小鼠的Bbc3(Puma)的BH3域。随后,一个loxP-PGKneo-loxP的片段被插入在第5外显子下游840bp的位置。之后,通过和Cre敲除转基因小鼠杂交,删除了neo cassette。(来源:J:153846)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
B6.Cg-Thy1a
Targeted
插入,基因内删除
--
1
--
3

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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