通过定点突变技术,用PCR替换了BH3域(在第5外显子),用的是小鼠的Bbc3(Puma)的BH3域。随后,一个loxP-PGKneo-loxP的片段被插入在第5外显子下游840bp的位置。之后,通过和Cre敲除转基因小鼠杂交,删除了neo cassette。(来源:J:153846)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count