germ line(生殖细胞)通过Cre介导的重组移除了myc标签、第45和46外显子,残余的蛋白仍保留着HA标签。通过 Western blot 分析确认了缺少C端的截短蛋白的表达(来源:J:153606)
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Germ line, cre mediated recombination removed the myc tag, exon 45 and exon 46. The truncated protein remains HA tagged. The expression of the truncated protein lacking the C-terminus was confirmed by western blot analysis. (J:153606)