在exon 2的上游插入了loxP位点,接着在exon 5的3'端插入了带有loxP位点的frt-flanked neo cassette。通过小鼠的生殖细胞,使用flp介导的重组去除了neo cassette,使得exons 2到5都被留下了loxP标记。(来源:J:153555)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count