在Nkx3-1基因的起始翻译位点插入了cre/ERT2-polyA序列,产生了无功能的等位基因。载体中还包括了ACE-cre/PolII-neo自我剔除的筛选 cassette用于阳性选择,以及PGK-tk(胸苷激酶) cassette用于阴性筛选。该载体被转染到了TC-1 ES细胞中,用于生成嵌合体。筛选 cassette通过雄性生殖系统的选择性剔除得以实现。(来源:J:153425)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S6/SvEvTac
Targeted
插入
--
1
--
21

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
Show/Hide columns
Phenotypes

References Literature

Title
PMID
Journal
Year
IF
No Data Found!
微信
信息比对
科研助手
使用教程
回到顶部