Exon 1被替换成带有lacZ、GFP和frt标记的neo cassette,缺失A和B形式的表达通过心脏、胫骨前肌和股四头肌的 Western blot分析得到了确认(来源:J:153150)
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Exon 1 was replaced with a floxed LacZ, GFP, and frt flanked neo cassette. The absence of expression of both the A and B form was confirmed by western blot analysis on heart, tibiallis anterior, and soleus extracts. (J:153150)