Tg(CAG-cat,-Tbx5)1Nemr

This transgene employs the "CAG promoter," which consists of the cytomegalovirus immediate early (CMV-EI) enhancer followed by a 1.3-kb DNA segment including the promoter, first exon and first intron of the chicken beta-actin gene, with the 3' splice junction sequence replaced by that of the rabbit hemoglobin beta gene. The promoter is separated from a mouse Tbx5 cDNA sequence for the Tbx5a isoform by a floxed chloramphenicol acetyltransferase (cat) gene with the SV40 small t intron and polyadenylation signal. Cre mediated excision of the chloramphenicol acetyltransferase results in Tbx5a expression. (J:67365, J:137268)