germ line(生殖细胞)通过Cre介导的重组移除了从2到4号的外显子以及neo cassette, 蛋白质表达的缺失通过在胚胎成纤维细胞提取物中进行 Western blot 分析得到了确认(来源:J:152821)
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Germ line, cre mediated recombination removed exons 2 through 4 and the neo cassette. The absence of protein expression was confirmed by western blot analysis on mouse embyonic fibroblast extracts. (J:152821)