从Myh6基因内含子中提取的一个DNA片段,包含micro RNA编码序列,通过同源重组被一个floxed neo cassette替换。随后,通过Cre介导的重组移除了neo cassette,留下一个在内含子中的loxP位点。PCR分析证实Myh6基因的剪接模式没有受到影响。Northern blot分析证实了纯合小鼠心脏中micro RNA的表达缺失。(来源:J:152696)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Not Specified
Targeted
基因内删除
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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