从3号外显子(可能是3号和4号,用(2?)表示)到5号,它们被替换了 neo抗药性基因座。RT-PCR和 Western blot的结果证实了转录本和蛋白质产品均不存在。(来源:J:152521)
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Exons 3(2?) through 5 were replaced with a neomycin resistance cassette. RT-PCR and Western blot analysis confirm the absence of both transcript and protein product. (J:152521)