germ line(生殖细胞)、通过Cre介导的重组被用来去除2到4号外显子。蛋白表达缺失通过在E10.5胚胎提取物中进行 Western blot 分析得到了确认。(来源:J:152259)
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Germ line, cre mediated recombination was used to remove exons 2 through 4. The absence of protein expression was confirmed by western blot analysis on E10.5 embryo extracts. (J:152259)