将一个新的neo cassette插入到了exon 3。通过在小鼠胚胎成纤维细胞提取物中进行 Western blot 分析,确认了没有蛋白质表达(来源:J:151543)。
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A neo cassette was inserted into exon 3. The absence of protein expression was confirmed by western blot analysis on mouse embryonic fibroblast extracts. (J:151543)