在exon 1中引入了突变,导致第33位的密码子从精氨酸(R33)变为谷氨酰胺(Q33)。随后在exon 1之后插入了loxP位点和frt标记的PGK-neomycin筛选元件。为了去除选择性片段,雄性小鼠与表达flp重组酶的雌性小鼠杂交。(来源:J:151367)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count