Gt(ROSA)26Sor^{tm8(RNAi:Crhr1)Rkuhn}

Using RMCE, an inserted hygro cassette was replaced with a neo cassette and a short hairpin RNA driven by the human U6 promoter that targets Crhr1. Expression of the shRNA results in an 80% reduction in endogenous Crhr1 transcript levels compared to in wild-type mice. (J:151155)