germ line(生殖细胞)、Cre介导的重组移除了5号和6号外显子,以及neo cassette(neo cassette未在文中提供具体含义,可保留原名)。蛋白质表达的缺失通过在小鼠胚胎成纤维细胞提取物中进行 Western blot 分析得到了确认。(来源:J:150995)
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Germ line, cre mediated recombination removes exons 5 and 6 along with the neo cassette.The absence of protein expression was confirmed by western blot analysis on mouse embryonic fibroblast extracts. (J:150995)