这个基因陷阱载体插入到了编码序列2上游的内含子1中,靠近起始翻译位点。通过胚胎干细胞提取物的 Western blot 分析,确实证实了蛋白质表达量的降低。 (Gene trap vector inserted into intron 1 upstream of exon 2, indicating the first translation initiation site, confirmed by Western blot on embryonic stem cell extracts.)
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The gene trap vector inserted into intron 1 upstream of exon 2, which contains the first translation initiation site. The reduction in protein expression was confirmed by western blot analysis on embryonic stem cell extracts.