Mcph1^{Gt(RRO608)Byg}

The vector, containing a splice acceptor sequence upstream of a reporter gene beta-galactosidase and neomycin phosphotransferase, was inserted into intron 12. This results in a truncated protein lacking 97 amino acids in the C-terminus. Insertion of the vector does not disrupt Angpt2, which is located in intron 12 of Mcph1. The expression of wild type mRNA is significantly reduced in all tissues, and the reporter is very weakly expressed, undetectable by western blot or in situ hybridization. (J:157933)