在基因座64,308,443至64,316,218处插入了pGT0Lxf这种带有β-geo报告基因的splice-acceptor序列和polyA尾巴的基因捕获载体,导致该基因包括exon 3的对应部分(nt 64,312,349-64,312,475)的缺失。E11.5同源突变胚胎的定量RT-PCR和Western blot结果显示没有mRNA和蛋白质表达。而X-Gal染色显示在E10.5杂合和纯合突变胚胎中lacZ的表达可检测到。插入点下游的31个基因片段均未表达(J:277387)。

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129P2/OlaHsd
Gene trapped
基因捕获载体的插入
--
1
--
3

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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