这个基因陷阱载体被插入在1号外显子和2号外显子之间。通过肺组织和小鼠胚胎成纤维细胞的RT-PCR和 Western blot分析,确实验证了转录和蛋白表达水平的降低。(来源:J:166002)
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The gene trap vector inserted between exon 1 and 2. Reduced transcript and protein expression was confirmed by QRT-PCR and western blot analysis on lung and mouse embryonic fibroblast extracts, respectively. (J:166002)