这个基因陷阱载体插入了第1外显子下游。通过 Western Blot 的分析,确认了长型蛋白表达缺失。短型蛋白的表达量降低到野生型的10%。(来源:J:177841)
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The gene trap vector inserted downstream of exon 1. The absence of protein expression of the long isoform was confirmed by western blot anaylsis. The expression of the short isoform is reduced to 10% of wild-type. (J:177841)