Mpdz^Gt(XG734)Byg

The insertional mutation by a gene-trapping vector, pGT1lxf, was designed to create an in-frame fusion between the 5' exons of the trapped gene and a reporter, geo (a fusion of beta-galactosidase and neomycin phosphotransferase II), and occurred in intron 11-12. The mutant yielded a fusion transcript containing exons 111 truncating the encoded protein after its third PDZ domain. Western blots of brain extracts from knockout homozygote pups confirmed the deletion and absence of full length encoded protein. (J:226456)