这个基因陷阱载体插入到了3号内含子。RT-PCR和 Western blot 的结果显示,来自纯合小鼠的胎盘中不含有RNA和蛋白质表达(来源:169234)。
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The gene trap vector inserted into intron 3. Rt-PCR and western blot analysis confirmed the absence of RNA and protein expression in placentas from homozygous mice. (J:169234)