在内皮2基因的第1内含子插入了一个基因陷阱 cassette,它包含了一个强的剪接接受器,连接着β-半乳糖苷酶的编码序列。 Western blot 检测证实了纯合突变小鼠中无蛋白表达。(来源:J:271138)
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A gene trap cassette inserted in intron 1 contained a strong splice acceptor from En2 fused to the coding sequence for beta-galactosidase. Western blot analysis confirmed the absence of protein expression in homozygous mutant mice. (J:271138)