Rfx2^{Gt(IST10638H11)Tigm}

A lacZ reporter poly A gene cassette with a splice acceptor was inserted into intron 1 of the gene before the ATG start codon in exon 2. Transcription at the locus should result in the splicing of exon 1 to the gene trap vector and eliminate the production of the endogenous transcript. RT-PCR analysis of total RNA from E9.5 embryos confirmed the absence of detectable mRNA in homozygous mutant samples. X-gal staining revealed that lacZ activity was present in ciliated tissues during mouse development including the node, the floor plate and the dorsal neural tube. (J:227136)