在编码序列的尾端,通过转暂时表达Cre重组酶将一个loxP引导的诺姆霉素插件插入第10个内含子,然后在第11个外显子末尾插入一个TAP标签 cassette。在靶向后,通过诺姆霉素插件的去除,对纯合小鼠进行多项生化和表型分析,结果显示TAP标签对p53功能蛋白的表达无影响。(来源:J:150816)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6Brd-Tyrc-Brd x 129S6/SvEvTac)F1
Targeted
插入
--
1
65
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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