Tg(Acta2-RAC1*G12V)33Pjgc

The cDNA coding for human RAC1 was mutated with a glycine 12 to valine substitution. The cDNA of RacCA, including its polyadenylation tail, was cloned within the Smp-8 plasmid that contained a 3.6 kb segment of the 5 kb-region of SMA promoter. The SMA promoter was used to induce selective overexpression of RacCA in SMCs. The purified fragment was microinjectedinto fertilized mouse eggs from FVB/N females, (J:150479)