Igh^{tm1.1(Tag)Rwhe}

A floxed neomycin resistance gene, a splice accepter, an internal ribosome entry site, and a SV40 large and small T antigen coding region were inserted in a reverse orientation between DQ52 and JH1 in the IgH locus. The neomycin gene was removed after targeting by crossing founder mice with transgenic mice expressing cre in the germline. SV40 T antigen transcripts were detected by RT-PCR in B cells. (J:150315)