第六外显子通过同源重组被替换成新插入的cassette。RT-PCR和 Western blot的结果证实,纯合小鼠的肾和精子中均未检测到表达。(来源:J:150175)
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Exon 6 was replaced with a neo cassette via homologous recombination. RT-PCR and western blot analysis confirmed the absence of expression in the kidney and kidney and sperm, respectively, from homozygous mice. (J:150175)