通过将携带敲除Gm73tm1Jxm基因为靶点的转基因小鼠与Cre转基因小鼠杂交,研究人员通过删除单个编码外显子创建了一个无功能等位基因。通过Southern blotting分析同源纯合组织提取物,确实证实了外显子的去除。(来源:J:149909)
Basic Information
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count