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中文
构建了一个靶向载体，其中在内含子7的NsiI酶切位点插入了上游的loxP序列，而在内含子9的BglII酶切位点插入了含荧光素抗药性基因的Neomycin抗性 cassette。这项操作在PC3 ES细胞中进行，这些细胞是Cre转录因子Tg(Prm-cre)70Og的纯合子，该转录因子在雄性生殖细胞中表达Cre重组酶。随后，Cre介导的重组只去除了Neomycin cassette，当雄性杂合子小鼠传代后。通过免疫印迹分析脾脏提取物，未发现表达受影响。来源：(J:149811)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
