这段经1到2号外显子的部分被替换成lacZ载体和一个可删除neo cassette。通过对初级软骨细胞进行 Western blot 分析,确认了没有蛋白质表达(来源:J:149382)。
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Part of exon 1 through exon 2 was replaced with a lacZ cassette and a floxed neo cassette. The absence of protein expression was confirmed by western blot analysis on primary chondrocytes. (J:149382)