外显子1被替换成一段人类CD4的剪接体cDNA以及一个带有floxed puromycin抗性标记的序列。通过睾丸提取物的 Western blot 检测,确实确认了蛋白表达的缺失。(来源:J:148942)
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Exon 1 was replaced with a truncated human CD4 cDNA and a floxed puromycin resistance cassette. The absence of protein expression was confirmed by western blot analysos of testes extracts. (J:148942)