通过同源重组,将一个floxed neo cassette插入了内皮9号染色体。 Western blot结果显示,纯合小鼠中,未刺激和刺激状态下巨噬细胞和内皮细胞的蛋白质表达水平确实降低了。(来源:J:148699)
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A floxed neo cassette was inserted into intron 9 via homologous recombination. Western blot analysis confirmed decreased protein expression in unstimulated and stimulated macrophages and endothelial cells from homozygous mice. (J:148699)