(来自期刊:148696)Exon 1到5被替换成lacZ基因和neo cassette,通过 Western blot 分析在杂合的胚胎成纤维细胞中证实了蛋白表达量的降低。
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Exon 1 through 5 was replaced with a lacZ gene and neo cassette. Reduced protein expression was confirmed in heterozygous mouse embryonic fibroblasts by western blot analysis. (J:148696)