Rffl^tm1Cota

The first coding exon of the gene is interrupted by a Betageo (beta-galactosidase/neomycin resistance fusion gene) reporter/selection cassette, resulting in the in-frame joining of the Betageo coding sequence to the mouse genomic sequence encoding the first 14 amino acids of the endogenous protein. Whereas wild-type mouse embryonic fibroblasts (MEFs) express RFFL mRNA at levels easily detected by RT-PCR analysis, MEFs from embryos homozygous for this mutation produce no detectable transcript. (J:148682)