Tg(Slc6a3-PARK2*Q311X)DXwy

A 200 kb mouse bacterial artificial chromosome (BAC) RP23-408F13 containing the entire 50 kb Slc6a3 gene (solute carrier family 6 (neurotransmitter transporter, dopamine), member 3; also called dopamine transporter (DAT)) and other genes in the approximately 100 kb of 5' and 40 kbp of 3' flanking sequences, was modified by inserting a parkin-Q311X cDNA sequence (FLAG-tagged mutant human parkin gene (PARK2; Parkinson disease (autosomal recessive, juvenile) 2, parkin) carrying the Q311X truncation associated with Turkish early-onset Parkinson's disease and followed by a polyA signal) into Slc6a3 exon 2 upstream of the translation initiation codon. This modified Slc6a3-parkin(Q311X) BAC was microinjected into fertilized FVB/NJ zygotes. Founder line D (found to harbor a single copy of the transgene) was established. Median expression of the human PARK2 is substantially lower than in line A. (J:146833)