通过同源重组,1.3kb包含exon 3的片段被替换为pgk-neo cassette。 Western blot结果显示,纯合小鼠大脑中没有检测到蛋白质表达(来源:J:148205)
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A 1.3 kb fragment including exon 3 was replaced with a pgk-neo cassette via homologous recombination. Western blot analysis confirmed the absence of protein expression in the brains of homozygous mice. (J:148205)