对于携带有Tcrbtm1Slek这一等位基因,且dbeta2-Jbeta2缺失的ES细胞,进行了第二次定向操作。预组装的dbeta1Jbeta1.1复合体被引入,它不包含N和P碱基,与基因组序列相比缺少一个C碱基。这个复合体取代了Dbeta1和Jbeta1.1的基因座。在Jbeta1.2和Jbeta1.3之间插入了floxed的诺卡因抗性基因和HindIII酶切位点。短暂表达的Cre重组酶移除了诺卡因基因座,留下HindIII酶切位点和一个loxP位点。新插入的HindIII酶切位点有助于区分这个等位基因上Tcrb的重排与Tcrbtm1Slek基因座的区别。(来源:J:147947)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Not Specified
Targeted
Insertion, Intergenic deletion
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4

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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